ABSTRACT Tinospora cordifolia plant extract was used for the green synthesis of AgFeO2 NPs, which were then characterised using FTIR (Fourier transform infrared spectroscopy), SEM (scanning electron microscopy), UV-vis spectroscopy, VSM (vibrating sample magnetometry), zeta potential, BET (Brunauer-Emmett-Teller), and XRD (X-ray diffraction) analysis. The notable XRD peaks observed at 550–600 cm−1 in the hybrid NPs can be attributed to the presence of iron oxide skeleton (-O-Fe), indicating the presence of AgFeO2 nanocrystals. Additionally, two peaks at approximately 526 and 486 cm−1 indicate the stretching vibrations of the Ag-O and Fe-O bonds within the AgFeO2 NPs, respectively. SEM analysis showed spherical nanoparticles forming irregular clusters with an average size of 19.43 nm. Vibrating Sample Magnetometer (VSM) analysis demonstrated that the AgFeO2 NPs exhibited superparamagnetic behaviour. This is characterised by an effective magnetisation of 61.57 emu/g, with low saturation magnetisation, magnetic remanence and non-zero coercivity making them suitable for magnetic applications. The zeta potential of −23.16 mV suggested NP stability due to electrostatic repulsion. UV-vis spectroscopy verified the existence of AgFeO2 NPs with an absorbance peak at 246 nm. BET analysis indicated that nanoparticles exhibited a large surface area of 3.4027 m2 g−1 and a nanoporous structure with an average pore size diameter of 3.1436 Å. 96% degradation of RB-13 dye was observed at pH 2 within contact time of 60 min, 50 mg concentration of catalyst and 10 ppm dye solution. The values of R2 = 0.96 and 0.94 confirm the validity of Pseudo’s first order and Langmuir-Hinshelwood’s kinetic models, respectively, for dye degradation. Anti-bacterial activity indicated that with increasing concentration of AgFeO2 NPs, the antibacterial activity becomes less because the time needed to detect bacteria was increased due to less active site availability for AgFeO2 NPs. The ascorbic acid consistently exhibits higher antioxidant activity at 82.25, 84.67, and 88.66% than Tinospora cordifolia leaf extract 51.34, 60.91, 74.02, and AgFeO2 NPs 61.54, 70.81, and 80.22 at 50, 75 and 100 μg/mL concentrations, respectively.